3543 pMIG Bcl-XL mut8
(Plasmid #8792)

• Depositing Lab: Stanley Korsmeyer
• Publication: Cheng et al Mol Cell 2001 Sep;8(3):705-11.

Backbone

• Vector backbone: pMIG
• Backbone size w/o insert (bp): 7000
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Bcl-XL
• Species: H. sapiens (human)
• Insert Size (bp): 700
• Mutation: G138A, R139L, I140N
• GenBank ID: NM_138578
• Entrez Gene: BCL2L1 (a.k.a. BCL-XL/S, BCL2L, BCLX, Bcl-X, PPP1R52)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bgl II (not destroyed)
• 5′ sequencing primer: pLXSN 5'

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that the GFP in this plasmid is not fused to gene. It is for selection purposes only.