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SIN-cPPT-H1-sgHTT1-U6-sgGFP2-7SK-sgCas9-PGK-mCherry-WPRE
(Plasmid #87919)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87919 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    HIV-1 SIN lentiviral transfer vector
  • Backbone size w/o insert (bp) 7600
  • Total vector size (bp) 10871
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgHTT1, shGFP2, sgCas9, cherry
  • Species
    Synthetic
  • Insert Size (bp)
    2434
  • Promoter H1, U6, 7SK, PGK

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer CACCGAACGCTGACGTCATCAACCCG
  • 3′ sequencing primer AGCAGCGTATCCACATAGCGTAAAAGGAGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SIN-cPPT-H1-sgHTT1-U6-sgGFP2-7SK-sgCas9-PGK-mCherry-WPRE was a gift from Nicole Déglon (Addgene plasmid # 87919 ; http://n2t.net/addgene:87919 ; RRID:Addgene_87919)

  • For your References section:

    The Self-Inactivating KamiCas9 System for the Editing of CNS Disease Genes. Merienne N, Vachey G, de Longprez L, Meunier C, Zimmer V, Perriard G, Canales M, Mathias A, Herrgott L, Beltraminelli T, Maulet A, Dequesne T, Pythoud C, Rey M, Pellerin L, Brouillet E, Perrier AL, du Pasquier R, Deglon N. Cell Rep. 2017 Sep 19;20(12):2980-2991. doi: 10.1016/j.celrep.2017.08.075. 10.1016/j.celrep.2017.08.075 PubMed 28930690
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