p-EGFP-C1-Flag-mFmr1(D)
(Plasmid
#87914)
-
PurposeExpresses mouse FMRP-EGFP fusion with phosphorylation mimic S499D.
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 87914 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepEGPF-C1
-
Backbone manufacturerBD Biosciences
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 6450
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameFmr1
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)1750
-
MutationS499D
-
Entrez GeneFmr1 (a.k.a. FMRP, Fmr-1)
-
Tags
/ Fusion Proteins
- Flag (N terminal on insert)
- eGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SacII (not destroyed)
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
p-EGFP-C1-Flag-mFmr1(D) was a gift from Stephanie Ceman (Addgene plasmid # 87914 ; http://n2t.net/addgene:87914 ; RRID:Addgene_87914) -
For your References section:
Identification and characterization of the methyl arginines in the fragile X mental retardation protein Fmrp. Stetler A, Winograd C, Sayegh J, Cheever A, Patton E, Zhang X, Clarke S, Ceman S. Hum Mol Genet. 2006 Jan 1;15(1):87-96. Epub 2005 Nov 30. 10.1093/hmg/ddi429 PubMed 16319129