p-EGFP-C1-Flag-mFmr1(A)
(Plasmid #87913)

• Purpose: Expresses mouse FMRP-EGFP fusion with S499A to prevent phosphorylation.
• Depositing Lab: Stephanie Ceman
• Publication: Stetler et al Hum Mol Genet. 2006 Jan 1;15(1):87-96. Epub 2005 Nov 30.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: BD Biosciences
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 6566
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Fmr1
• Species: M. musculus (mouse)
• Insert Size (bp): 1866
• Mutation: S499A
• Entrez Gene: Fmr1 (a.k.a. FMRP, Fmr-1)
• Tags / Fusion Proteins:
  • Flag (N terminal on insert)
  • eGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SacII (not destroyed)
• 5′ sequencing primer: EGFP-C

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry