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Addgene

pUC57 DYSF, alt exon 1, 5a, 17, 40a
(Plasmid #87895)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87895 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57 Kan
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DYSF, alt exon 1, 5a, 17, 40a
  • Species
    H. sapiens (human)
  • Mutation
    alternative isoforms, see comments
  • Entrez Gene
    DYSF (a.k.a. FER1L1, LGMD2B, LGMDR2, MMD1)

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There are alternative isoforms of the dysferlin protein. The insert contained in this plasmid is described as such: 1. Dysferlin can utilize exon 1 or alternative exon 1a to build a full protein. Therefore, which exon 1 has been included in the construct is noted. 2. Exons 5a, 17 and 40a are optional exons, so if the exon is listed, it is included in the name. 3. The remainder of the exons are not optional, and are included in all constructs.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC57 DYSF, alt exon 1, 5a, 17, 40a was a gift from Jain Foundation (Addgene plasmid # 87895 ; http://n2t.net/addgene:87895 ; RRID:Addgene_87895)
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