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Addgene

3540 pMIG Bid p15
(Plasmid #8789)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8789 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMIG
  • Backbone size w/o insert (bp) 7000
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bid p15
  • Alt name
    tBid
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    410
  • GenBank ID
    NM_007544
  • Entrez Gene
    Bid (a.k.a. 2700049M22Rik)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl II (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer pLXSN 5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

tBid can be cut out by EcoRI

Note that the GFP in this plasmid is not fused to gene. It is for selection purposes only.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3540 pMIG Bid p15 was a gift from Stanley Korsmeyer (Addgene plasmid # 8789 ; http://n2t.net/addgene:8789 ; RRID:Addgene_8789)

  • For your References section:

    BCL-2, BCL-X(L) sequester BH3 domain-only molecules preventing BAX- and BAK-mediated mitochondrial apoptosis. Cheng EH, Wei MC, Weiler S, Flavell RA, Mak TW, Lindsten T, Korsmeyer SJ. Mol Cell 2001 Sep;8(3):705-11. 10.1016/S1097-2765(01)00320-3 PubMed 11583631
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