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Addgene

His 3C Kinase dead MKK6 1-334
(Plasmid #87882)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87882 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET28 NKI LIC
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MKK6
  • Species
    H. sapiens (human)
  • Mutation
    K82A, kinase dead mutant
  • Entrez Gene
    MAP2K6 (a.k.a. MAPKK6, MEK6, MKK6, PRKMK6, SAPKK-3, SAPKK3)
  • Promoter T7
  • Tags / Fusion Proteins
    • 6xHis (N terminal on backbone)
    • 3C protease site (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    His 3C Kinase dead MKK6 1-334 was a gift from Peter Mace (Addgene plasmid # 87882 ; http://n2t.net/addgene:87882 ; RRID:Addgene_87882)
  • For your References section:

    Structural basis of autoregulatory scaffolding by apoptosis signal-regulating kinase 1. Weijman JF, Kumar A, Jamieson SA, King CM, Caradoc-Davies TT, Ledgerwood EC, Murphy JM, Mace PD. Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):E2096-E2105. doi: 10.1073/pnas.1620813114. Epub 2017 Feb 27. 10.1073/pnas.1620813114 PubMed 28242696