His 3C Kinase dead MKK6 1-334
(Plasmid
#87882)
-
PurposeE. coli expression of MKK6 kinase dead
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 87882 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepET28 NKI LIC
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameMKK6
-
SpeciesH. sapiens (human)
-
MutationK82A, kinase dead mutant
-
Entrez GeneMAP2K6 (a.k.a. MAPKK6, MEK6, MKK6, PRKMK6, SAPKK-3, SAPKK3)
- Promoter T7
-
Tags
/ Fusion Proteins
- 6xHis (N terminal on backbone)
- 3C protease site (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
His 3C Kinase dead MKK6 1-334 was a gift from Peter Mace (Addgene plasmid # 87882 ; http://n2t.net/addgene:87882 ; RRID:Addgene_87882) -
For your References section:
Structural basis of autoregulatory scaffolding by apoptosis signal-regulating kinase 1. Weijman JF, Kumar A, Jamieson SA, King CM, Caradoc-Davies TT, Ledgerwood EC, Murphy JM, Mace PD. Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):E2096-E2105. doi: 10.1073/pnas.1620813114. Epub 2017 Feb 27. 10.1073/pnas.1620813114 PubMed 28242696
