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PurposeExpress a EGFP-human GABARAP in mammalian cells
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 87871 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerCLONTECH
- Backbone size w/o insert (bp) 4600
- Total vector size (bp) 5067
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGABARAP
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SpeciesH. sapiens (human)
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Insert Size (bp)354
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Entrez GeneGABARAP (a.k.a. ATG8A, GABARAP-a, MM46)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-hGABARAP was a gift from Eiki Kominami & Isei Tanida (Addgene plasmid # 87871 ; http://n2t.net/addgene:87871 ; RRID:Addgene_87871) -
For your References section:
The human homolog of Saccharomyces cerevisiae Apg7p is a Protein-activating enzyme for multiple substrates including human Apg12p, GATE-16, GABARAP, and MAP-LC3. Tanida I, Tanida-Miyake E, Ueno T, Kominami E. J Biol Chem. 2001 Jan 19;276(3):1701-6. Epub 2000 Nov 28. 10.1074/jbc.C000752200 PubMed 11096062