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Addgene

pEGFP-hGATE-16
(Plasmid #87870)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87870 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    CLONTECH
  • Backbone size w/o insert (bp) 4600
  • Total vector size (bp) 5067
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GATE-16
  • Alt name
    GEF2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    354
  • Entrez Gene
    GABARAPL2 (a.k.a. ATG8, ATG8C, GATE-16, GATE16, GEF-2, GEF2)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-hGATE-16 was a gift from Eiki Kominami & Isei Tanida (Addgene plasmid # 87870 ; http://n2t.net/addgene:87870 ; RRID:Addgene_87870)

  • For your References section:

    The human homolog of Saccharomyces cerevisiae Apg7p is a Protein-activating enzyme for multiple substrates including human Apg12p, GATE-16, GABARAP, and MAP-LC3. Tanida I, Tanida-Miyake E, Ueno T, Kominami E. J Biol Chem. 2001 Jan 19;276(3):1701-6. Epub 2000 Nov 28. 10.1074/jbc.C000752200 PubMed 11096062
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