pCMV-hATG7CS
(Plasmid #87868)

• Purpose: Express a active-site mutant human Atg7/Apg7 C572S in mammalian cells
• Depositing Labs: Eiki Kominami, Isei Tanida
• Publication: Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6. Epub 2000 Nov 28.

Backbone

• Vector backbone: pEGFP-N1
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 6000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ATG7 C572S mutant
• Alt name: autophagy-related gene 7 C572S mutant
• Alt name: APG7 C572S mutant
• Alt name: ATG7 active site-mutant
• Species: H. sapiens (human)
• Insert Size (bp): 2123
• Mutation: C572S mutant
• GenBank ID: AF094516
• Entrez Gene: ATG7 (a.k.a. APG7-LIKE, APG7L, GSA7, SCAR31)
• Promoter: CMV

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: ATGTCGTAACAACTCCGCC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Based on pCMV-hATG7wt, an active site Cys572 within human ATG7 was changed to Ser by site-directed mutagenesis. An unlinked, promoterless, EGFP is also present in the backbone of the plasmid.