pEGFPN1-N-gamma-tubulin (334-449)
(Plasmid #87859)

• Purpose: Fluorescent fragment of gamma-tubulin containing gamma-tubulin DNA binding domain and NLS
• Depositing Lab: Maria Alvarado-Kristensson
• Publication: Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 10.1096/fj.11-187484. Epub 2011 Jul 25.

Backbone

• Vector backbone: pEGFP-N1
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: gamma-tubulin 1
• Alt name: TUBG1
• Species: H. sapiens (human)
• Insert Size (bp): 345
• Mutation: fragment from aa 334 to 449
• GenBank ID: NM_001070.4
• Entrez Gene: TUBG1 (a.k.a. CDCBM4, GCP-1, TUBG, TUBGCP1)
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The TUBG1 was originally cloned by Dr. Jiri Bartek, I subclonned the c-terminal region into the pEGFN1 vector.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The C-terminal is a sh-gamma-tubulin resistant gene. C 234 and 240 are mutated to T