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Addgene

pFLP-hyg
(Plasmid #87831)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87831 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCP20
  • Backbone manufacturer
    Yale Coli Genetic Stock Center
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 7000
  • Modifications to backbone
    1. replication origin is p15a ts 2. cat and amp resistance markers in pCP20 replaced with hyg resistance marker
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin, 200 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hyg
  • Insert Size (bp)
    1040
  • Mutation
    None
  • Tag / Fusion Protein
    • None

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFLP-hyg was a gift from Pep Charusanti (Addgene plasmid # 87831 ; http://n2t.net/addgene:87831 ; RRID:Addgene_87831)

  • For your References section:

    Capsule deletion via a lambda-Red knockout system perturbs biofilm formation and fimbriae expression in Klebsiella pneumoniae MGH 78578. Huang TW, Lam I, Chang HY, Tsai SF, Palsson BO, Charusanti P. BMC Res Notes. 2014 Jan 8;7:13. doi: 10.1186/1756-0500-7-13. 10.1186/1756-0500-7-13 PubMed 24398052
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