pACBSR-hyg
(Plasmid #87830)

• Purpose: Expresses lambda-red system with hyg selection marker
• Depositing Lab: Pep Charusanti
• Publication: Huang et al BMC Res Notes. 2014 Jan 8;7:13. doi: 10.1186/1756-0500-7-13.

Backbone

• Vector backbone: pACBSR, which is based on pBAD
• Backbone manufacturer: N/A
• Backbone size w/o insert (bp): 6300
• Total vector size (bp): 7300
• Modifications to backbone: 1. camR in pACBSR replace with hygR 2. replication origin is p15a ts
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Hygromycin, 200 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Growth instructions: Might be possible to grow at 37C, but 30C works fine
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: hyg
• Insert Size (bp): 1040
• Mutation: None
• Promoter: bla
• Tag / Fusion Protein:
  • None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BgI II (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: AGATCTCGGGGAAATGTGCGCGGAAC
• 3′ sequencing primer: CTCGAGGTATATATGAGTAAACTTGGTCTG

Resource Information

• Supplemental Documents:
  • Full K. pneumoniae knockout protocol

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry