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Addgene

3501 pcDNA3 Bid p15 mIII.4
(Plasmid #8781)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8781 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bid p15 mIII.4
  • Alt name
    Bid
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1200
  • GenBank ID
    NM_007544
  • Entrez Gene
    Bid (a.k.a. 2700049M22Rik)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3501 pcDNA3 Bid p15 mIII.4 was a gift from Stanley Korsmeyer (Addgene plasmid # 8781 ; http://n2t.net/addgene:8781 ; RRID:Addgene_8781)

  • For your References section:

    tBID, a membrane-targeted death ligand, oligomerizes BAK to release cytochrome c. Wei MC, Lindsten T, Mootha VK, Weiler S, Gross A, Ashiya M, Thompson CB, Korsmeyer SJ. Genes Dev 2000 Aug 15;14(16):2060-71. PubMed 10950869
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