pTNS1
(Plasmid #87801)

• Purpose: (Empty Backbone) For constructing NusA-SUMO-fusion proteins with N-terminal His tag and C-terminal strep II tag using "blunt-end" cloning.
• Depositing Lab: Yu-Zhu Zhang
• Publication: Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167. doi: 10.1007/s12033-017-9998-6.

Backbone

• Vector backbone: pRSFduet-1
• Backbone manufacturer: EMD Millipore
• Vector type: Bacterial Expression
• Tags / Fusion Proteins:
  • 6xHis (N terminal on backbone)
  • Strep II (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tags / Fusion Proteins:
  • 6xHis (N terminal on backbone)
  • Strep II (C terminal on backbone)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The constructs contain a DNA sequence which encodes a mutant sumo from yeast. I had the coding sequence synthesized by a commercial vendor.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Additional reference: Zhang, Y.; Du, W.X.; Fregevu, C.; Kothary, M.H.; Harden, L; McHugh, T.H. Expression, purification, and characterization of almond (Prunus dulcis) allergen Pru du 4. J. Agric Food Chem 2014, 62, 12695-700. PMID: 25437489