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Addgene

3494 pcDNA3 Bax
(Plasmid #8779)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8779 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bax
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    930
  • GenBank ID
    NM_007527
  • Entrez Gene
    Bax

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3494 pcDNA3 Bax was a gift from Stanley Korsmeyer (Addgene plasmid # 8779 ; http://n2t.net/addgene:8779 ; RRID:Addgene_8779)

  • For your References section:

    Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Oltvai ZN, Milliman CL, Korsmeyer SJ. Cell 1993 Aug 27;74(4):609-19. 10.1016/0092-8674(93)90509-O PubMed 8358790
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