pSLIK 3XFLAG-MKK7-EE-NLS hygro
(Plasmid #87780)

• Purpose: Inducible expression of constitutively active mutant MKK7 with a C-terminal NLS sequence
• Depositing Lab: Kevin Janes
• Publication: Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl 1):S244-S262. doi: 10.1074/mcp.O116.063487. Epub 2017 Feb 7.

Backbone

• Vector backbone: pSLIK-Hygro
• Backbone manufacturer: Addgene #25737
• Backbone size w/o insert (bp): 13918
• Total vector size (bp): 13804
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MKK7
• Species: M. musculus (mouse)
• Insert Size (bp): 1155
• GenBank ID: NM_001042557
• Entrez Gene: Map2k7 (a.k.a. 5930412N11Rik, JNKK 2, Jnkk, Jnkk2, MAPKK 7, MEK 7, Mapkk7, Mek7, Mkk7, Prkm, Prkmk7, sek, sek2)
• Tags / Fusion Proteins:
  • 3xFLAG (N terminal on insert)
  • NLS (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: hUBCpro-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry