pSLIK 3XFLAG-MKK4-EE-NLS hygro
(Plasmid
#87779)
-
PurposeInducible expression of constitutively active mutant MKK4 with a C-terminal NLS sequence
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 87779 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepSLIK-Hygro
-
Backbone manufacturerAddgene #25737
- Backbone size w/o insert (bp) 13918
- Total vector size (bp) 13855
-
Vector typeMammalian Expression, Lentiviral
-
Selectable markersHygromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameMKK4
-
Insert Size (bp)1131
-
GenBank IDNM_003010
-
Tags
/ Fusion Proteins
- 3xFLAG (N terminal on insert)
- NLS (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer hUBCpro-R (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pSLIK 3XFLAG-MKK4-EE-NLS hygro was a gift from Kevin Janes (Addgene plasmid # 87779 ; http://n2t.net/addgene:87779 ; RRID:Addgene_87779) -
For your References section:
Profiling Subcellular Protein Phosphatase Responses to Coxsackievirus B3 Infection of Cardiomyocytes. Shah M, Smolko CM, Kinicki S, Chapman ZD, Brautigan DL, Janes KA. Mol Cell Proteomics. 2017 Apr;16(4 suppl 1):S244-S262. doi: 10.1074/mcp.O116.063487. Epub 2017 Feb 7. 10.1074/mcp.O116.063487 PubMed 28174228