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pEN_TTmiRc2 3XFLAG-MKK4-EE-NLS
(Plasmid #87776)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87776 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEN_TTmiRc2 3xFLAG
  • Backbone manufacturer
    Addgene #83274
  • Backbone size w/o insert (bp) 4496
  • Total vector size (bp) 4989

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MKK4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1131
  • GenBank ID
    none
  • Entrez Gene
    MAP2K4 (a.k.a. JNKK, JNKK1, MAPKK4, MEK4, MKK4, PRKMK4, SAPKK-1, SAPKK1, SEK1, SERK1, SKK1)
  • Tags / Fusion Proteins
    • 3xFLAG (N terminal on backbone)
    • NLS (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Spe1 (not destroyed)
  • 3′ cloning site Mfe1 (not destroyed)
  • 5′ sequencing primer pCEP-FWD
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEN_TTmiRc2 3XFLAG-MKK4-EE-NLS was a gift from Kevin Janes (Addgene plasmid # 87776 ; http://n2t.net/addgene:87776 ; RRID:Addgene_87776)

  • For your References section:

    Profiling Subcellular Protein Phosphatase Responses to Coxsackievirus B3 Infection of Cardiomyocytes. Shah M, Smolko CM, Kinicki S, Chapman ZD, Brautigan DL, Janes KA. Mol Cell Proteomics. 2017 Apr;16(4 suppl 1):S244-S262. doi: 10.1074/mcp.O116.063487. Epub 2017 Feb 7. 10.1074/mcp.O116.063487 PubMed 28174228
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