pLX302-NIPP1-V5 puro
(Plasmid #87775)

• Purpose: Expresses V5 tagged human NIPP1
• Depositing Lab: Kevin Janes
• Publication: Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl 1):S244-S262. doi: 10.1074/mcp.O116.063487. Epub 2017 Feb 7.

Backbone

• Vector backbone: pLX302
• Backbone size w/o insert (bp): 9573
• Total vector size (bp): 8545
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: NIPP1
• Alt name: PPP1R8
• Species: H. sapiens (human)
• Insert Size (bp): 629
• GenBank ID: NM_138558.2
• Entrez Gene: PPP1R8 (a.k.a. ARD-1, ARD1, NIPP-1, NIPP1, PRO2047)
• Tag / Fusion Protein:
  • V5 (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: WPRE-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Human ORFeome #6578

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry