3486 pBABE Bid p22 GFP
(Plasmid
#8777)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8777 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBABE-puro
- Backbone size w/o insert (bp) 5100
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBid p22
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Alt nameBid
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SpeciesM. musculus (mouse)
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Insert Size (bp)1200
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GenBank IDNM_007544
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Entrez GeneBid (a.k.a. 2700049M22Rik)
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Tag
/ Fusion Protein
- GFP
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pBABE 5' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
3486 pBABE Bid p22 GFP was a gift from Stanley Korsmeyer (Addgene plasmid # 8777 ; http://n2t.net/addgene:8777 ; RRID:Addgene_8777) -
For your References section:
Posttranslational N-myristoylation of BID as a molecular switch for targeting mitochondria and apoptosis. Zha J, Weiler S, Oh KJ, Wei MC, Korsmeyer SJ. Science 2000 Dec 1;290(5497):1761-5. 10.1126/science.290.5497.1761 PubMed 11099414