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Addgene

pCK300
(Plasmid #87766)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87766 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJ404
  • Backbone manufacturer
    DNA2.0
  • Backbone size (bp) 4000
  • Modifications to backbone
    T5 promoter removed
  • Vector type
    Bacterial Expression, Synthetic Biology
  • Promoter None

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCK300 was a gift from John Heap (Addgene plasmid # 87766 ; http://n2t.net/addgene:87766 ; RRID:Addgene_87766)

  • For your References section:

    Synthetic Chemical Inducers and Genetic Decoupling Enable Orthogonal Control of the rhaBAD Promoter. Kelly CL, Liu Z, Yoshihara A, Jenkinson SF, Wormald MR, Otero J, Estevez A, Kato A, Marqvorsen MH, Fleet GW, Estevez RJ, Izumori K, Heap JT. ACS Synth Biol. 2016 Oct 21;5(10):1136-1145. Epub 2016 Jun 21. 10.1021/acssynbio.6b00030 PubMed 27247275
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