EB2317
(Plasmid
#87754)
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PurposetetR-mTurqoise2 cloned between BamHI and EcoRI of pAM2991
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 87754 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAm2991
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Backbone manufacturerSusan Golden lab
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTetR-mTurq2
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene NGS results found that the last 10 C-terminal residues of mTurq2 (ITLGMDELYK) have been replaced by MQAWLFWRMREDFQPDTD. Depositor states that this was the construct used in experiments and should be functional
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EB2317 was a gift from Erin O'Shea (Addgene plasmid # 87754 ; http://n2t.net/addgene:87754 ; RRID:Addgene_87754) -
For your References section:
Cyanobacteria Maintain Constant Protein Concentration despite Genome Copy-Number Variation. Zheng XY, O'Shea EK. Cell Rep. 2017 Apr 18;19(3):497-504. doi: 10.1016/j.celrep.2017.03.067. 10.1016/j.celrep.2017.03.067 PubMed 28423314