3440 pcDNA3 Bid p22
(Plasmid
#8774)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8774 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBid p22 wt
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Alt nameBid
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SpeciesM. musculus (mouse)
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Insert Size (bp)600
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GenBank IDNM_007544
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Entrez GeneBid (a.k.a. 2700049M22Rik)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
3440 pcDNA3 Bid p22 was a gift from Stanley Korsmeyer (Addgene plasmid # 8774 ; http://n2t.net/addgene:8774 ; RRID:Addgene_8774) -
For your References section:
Posttranslational N-myristoylation of BID as a molecular switch for targeting mitochondria and apoptosis. Zha J, Weiler S, Oh KJ, Wei MC, Korsmeyer SJ. Science 2000 Dec 1;290(5497):1761-5. 10.1126/science.290.5497.1761 PubMed 11099414