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Addgene

3413 pBF339 Bcl-XL
(Plasmid #8773)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8773 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBF339
  • Backbone size w/o insert (bp) 6680
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bcl-XL
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    700
  • Entrez Gene
    Bcl2l1 (a.k.a. Bcl(X)L, Bcl-XL, Bcl2l, BclX, bcl-x, bcl2-L-1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer HA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

cDNA encoding murine BCL-XL into EcoRI site directly downstream of yeast ADH1 promoter, also 2um origin of replication

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3413 pBF339 Bcl-XL was a gift from Stanley Korsmeyer (Addgene plasmid # 8773 ; http://n2t.net/addgene:8773 ; RRID:Addgene_8773)

  • For your References section:

    Biochemical and genetic analysis of the mitochondrial response of yeast to BAX and BCL-X(L). Gross A, Pilcher K, Blachly-Dyson E, Basso E, Jockel J, Bassik MC, Korsmeyer SJ, Forte M. Mol Cell Biol 2000 May;20(9):3125-36. 10.1128/MCB.20.9.3125-3136.2000 PubMed 10757797
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