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Addgene

pCMV-Tag/E112K with 5'UTR
(Plasmid #87723)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87723 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-Tag
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4300
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SPAST
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2027
  • Mutation
    E112K
  • GenBank ID
    NM_014946
  • Entrez Gene
    SPAST (a.k.a. ADPSP, FSP2, SPG4)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoICRI (destroyed during cloning)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cloning of spastin cDNA into pCMV-Tag EcoICRI and BamHI sites removed myc tag. To detect spastin, use an anti-spastin antibody.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-Tag/E112K with 5'UTR was a gift from Peter Baas (Addgene plasmid # 87723 ; http://n2t.net/addgene:87723 ; RRID:Addgene_87723)

  • For your References section:

    Evaluation of loss of function as an explanation for SPG4-based hereditary spastic paraplegia. Solowska JM, Garbern JY, Baas PW. Hum Mol Genet. 2010 Jul 15;19(14):2767-79. doi: 10.1093/hmg/ddq177. Epub 2010 Apr 29. 10.1093/hmg/ddq177 PubMed 20430936
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