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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 8772 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBM272
- Backbone size w/o insert (bp) 8259
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBcl-XL
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SpeciesM. musculus (mouse)
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Insert Size (bp)700
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GenBank IDNM_138578
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Entrez GeneBcl2l1 (a.k.a. Bcl(X)L, Bcl-XL, Bcl2l, BclX, bcl-x, bcl2-L-1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer Gal10pro-F (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Gal10 promoter, also CEN4 and ARS1
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
3410 pBM272 Bcl-XL was a gift from Stanley Korsmeyer (Addgene plasmid # 8772 ; http://n2t.net/addgene:8772 ; RRID:Addgene_8772) -
For your References section:
Biochemical and genetic analysis of the mitochondrial response of yeast to BAX and BCL-X(L). Gross A, Pilcher K, Blachly-Dyson E, Basso E, Jockel J, Bassik MC, Korsmeyer SJ, Forte M. Mol Cell Biol 2000 May;20(9):3125-36. 10.1128/MCB.20.9.3125-3136.2000 PubMed 10757797
Map uploaded by the depositor.