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Addgene

3410 pBM272 Bcl-XL
(Plasmid #8772)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8772 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBM272
  • Backbone size w/o insert (bp) 8259
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bcl-XL
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    700
  • GenBank ID
    NM_138578
  • Entrez Gene
    Bcl2l1 (a.k.a. Bcl(X)L, Bcl-XL, Bcl2l, BclX, bcl-x, bcl2-L-1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer Gal10pro-F
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gal10 promoter, also CEN4 and ARS1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3410 pBM272 Bcl-XL was a gift from Stanley Korsmeyer (Addgene plasmid # 8772 ; http://n2t.net/addgene:8772 ; RRID:Addgene_8772)
  • For your References section:

    Biochemical and genetic analysis of the mitochondrial response of yeast to BAX and BCL-X(L). Gross A, Pilcher K, Blachly-Dyson E, Basso E, Jockel J, Bassik MC, Korsmeyer SJ, Forte M. Mol Cell Biol 2000 May;20(9):3125-36. 10.1128/MCB.20.9.3125-3136.2000 PubMed 10757797