Lyn-FLINC-EKAR1(TA)
(Plasmid #87708)

• Purpose: Negative-control mutant of Lyn-FLINC-EKAR1.
• Depositing Lab: Jin Zhang
• Publication: Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi: 10.1038/nmeth.4221. Epub 2017 Mar 13.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 7410
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Lyn-FLINC-EKAR1(TA)
• Alt name: Lyn-Dronpa-WW-EV-ERKsub(TA)-TagRFPT
• Species: S. cerevisiae (budding yeast), Synthetic
• Insert Size (bp): 1956
• Mutation: Target Thr residue in substrate domain mutated to Ala.
• Promoter: CMV
• Tag / Fusion Protein:
  • N-terminal targeting sequence from Lyn kinase (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7 forward
• 3′ sequencing primer: BGH reverse

Resource Information

• Supplemental Documents:
  • pcDNA3-Lyn-FLINC-EKAR1(TA).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry