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Addgene

pET-MBP-NLS-Geo_st
(Plasmid #87703)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87703 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GeoCas9
  • Species
    Geobacillus stearothermophilus
  • Insert Size (bp)
    3349
  • GenBank ID
    OAO79865.1
  • Tag / Fusion Protein
    • 10xHis-MBP-TEV (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GATGAAGCCCTGAAAGACGCGCAG
  • 3′ sequencing primer CTA GTT ATT GCT CAG CGG T
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please see http://biorxiv.org/content/early/2017/05/16/138867 for the preprint publication describing this construct.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-MBP-NLS-Geo_st was a gift from Jennifer Doudna (Addgene plasmid # 87703 ; http://n2t.net/addgene:87703 ; RRID:Addgene_87703)
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