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Addgene

pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2
(Plasmid #87694)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87694 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ERT2-PhoCl-Cre-PhoCl-ERT2
  • Species
    Synthetic
  • Promoter Chicken beta actin

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    This vector plasmid was modified from pCAG-ERT2CreERT2 (Connie Cepko lab, Addgene plasmid # 13777)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There's a Leu to Val mismatch between the reference sequence and Addgene's quality control results. The change is in the linker region between PhoCl and ERT2 and should not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2 was a gift from Robert Campbell (Addgene plasmid # 87694 ; http://n2t.net/addgene:87694 ; RRID:Addgene_87694)

  • For your References section:

    Optogenetic control with a photocleavable protein, PhoCl. Zhang W, Lohman AW, Zhuravlova Y, Lu X, Wiens MD, Hoi H, Yaganoglu S, Mohr MA, Kitova EN, Klassen JS, Pantazis P, Thompson RJ, Campbell RE. Nat Methods. 2017 Mar 13. doi: 10.1038/nmeth.4222. 10.1038/nmeth.4222 PubMed 28288123
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