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PurposeExpress ERT2-PhoCl-Gal4VP16-PhoCl-ERT2 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 87693 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameERT2-PhoCl-Gal4VP16-PhoCl-ERT2
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SpeciesSynthetic
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byThis vector plasmid was modified from pCAG-ERT2CreERT2 (Connie Cepko lab, Addgene plasmid # 13777)
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-ERT2-PhoCl-Gal4VP16-PhoCl-ERT2 was a gift from Robert Campbell (Addgene plasmid # 87693 ; http://n2t.net/addgene:87693 ; RRID:Addgene_87693) -
For your References section:
Optogenetic control with a photocleavable protein, PhoCl. Zhang W, Lohman AW, Zhuravlova Y, Lu X, Wiens MD, Hoi H, Yaganoglu S, Mohr MA, Kitova EN, Klassen JS, Pantazis P, Thompson RJ, Campbell RE. Nat Methods. 2017 Mar 13. doi: 10.1038/nmeth.4222. 10.1038/nmeth.4222 PubMed 28288123