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Addgene

3349 pSFFV-neo Bcl-2
(Plasmid #8769)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8769 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSFFV-neo
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 7200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bcl-2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    720
  • GenBank ID
    NM_000633
  • Entrez Gene
    BCL2 (a.k.a. Bcl-2, PPP1R50)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SFFV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

ORF contains SacI and BamHI sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3349 pSFFV-neo Bcl-2 was a gift from Stanley Korsmeyer (Addgene plasmid # 8769 ; http://n2t.net/addgene:8769 ; RRID:Addgene_8769)

  • For your References section:

    BCL-2 is phosphorylated and inactivated by an ASK1/Jun N-terminal protein kinase pathway normally activated at G(2)/M. Yamamoto K, Ichijo H, Korsmeyer SJ. Mol Cell Biol 1999 Dec;19(12):8469-78. PubMed 10567572
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