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Addgene

pBAD/HisB-PhoCl
(Plasmid #87688)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87688 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD/HisB
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PhoCl
  • Species
    Synthetic
  • Insert Size (bp)
    744
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD/HisB-PhoCl was a gift from Robert Campbell (Addgene plasmid # 87688 ; http://n2t.net/addgene:87688 ; RRID:Addgene_87688)

  • For your References section:

    Optogenetic control with a photocleavable protein, PhoCl. Zhang W, Lohman AW, Zhuravlova Y, Lu X, Wiens MD, Hoi H, Yaganoglu S, Mohr MA, Kitova EN, Klassen JS, Pantazis P, Thompson RJ, Campbell RE. Nat Methods. 2017 Mar 13. doi: 10.1038/nmeth.4222. 10.1038/nmeth.4222 PubMed 28288123
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