pFLAG-CMV2 MAML LHHLL
(Plasmid #87681)

• Purpose: Mammalian expression vector for Maml1 with LHHLL mutation
• Depositing Lab: Karl Munger
• Publication: Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171. doi: 10.1371/journal.ppat.1006171.

Backbone

• Vector backbone: pFLAG-CMV2
• Backbone manufacturer: Sigma
• Backbone size w/o insert (bp): 4679
• Total vector size (bp): 7727
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MAML1
• Species: H. sapiens (human)
• Insert Size (bp): 3048
• Mutation: Mutate 1010,1011 from DD to HH
• Entrez Gene: MAML1 (a.k.a. Mam-1, Mam1)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: CMV forward

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Original template DNA was a gift of J. Aster.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Additional mutation G162C within the MAML1 translation was in the original CMV2 Flag MAML plasmid we were given by the J. Aster lab