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Addgene

pLentiN HPV8 E6 K142A
(Plasmid #87680)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87680 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti6.3/V5 DEST
  • Backbone size w/o insert (bp) 9387
  • Total vector size (bp) 8600
  • Vector type
    Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HPV 8E6
  • Species
    HPV 8
  • Mutation
    K142A
  • GenBank ID
    M12737.1
  • Promoter CMV
  • Tags / Fusion Proteins
    • Flag (N terminal on backbone)
    • HA (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLentiN HPV8 E6 K142A was a gift from Karl Munger (Addgene plasmid # 87680 ; http://n2t.net/addgene:87680 ; RRID:Addgene_87680)

  • For your References section:

    Cutaneous HPV8 and MmuPV1 E6 Proteins Target the NOTCH and TGF-beta Tumor Suppressors to Inhibit Differentiation and Sustain Keratinocyte Proliferation. Meyers JM, Uberoi A, Grace M, Lambert PF, Munger K. PLoS Pathog. 2017 Jan 20;13(1):e1006171. doi: 10.1371/journal.ppat.1006171. PPATHOGENS-D-16-02140 [pii] PubMed 28107544
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