hDAGLa-V5
(Plasmid
#87676)
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PurposeExpresses human DAGL? protein with intracellular V5 tag located on the C-terminal
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 87676 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA6.2-DEST/V7
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 7341
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Vector typeMammalian Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHuman Diacylglycerol Lipase Alpha
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Alt namehDAGLa
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SpeciesH. sapiens (human)
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Insert Size (bp)3126
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Entrez GeneDAGLA (a.k.a. C11orf11, DAGL(ALPHA), DAGLALPHA, NSDDR)
- Promoter T7
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Tag
/ Fusion Protein
- V5 tag (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer V5 Rev Priming Site (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hDAGLa-V5 was a gift from Pat Doherty (Addgene plasmid # 87676 ; http://n2t.net/addgene:87676 ; RRID:Addgene_87676) -
For your References section:
A novel live cell assay to measure diacylglycerol lipase alpha activity. Singh PK, Markwick R, Howell FV, Williams G, Doherty P. Biosci Rep. 2016 May 6;36(3). pii: e00331. doi: 10.1042/BSR20160073. Print 2016 Jun. 10.1042/BSR20160073 PubMed 27013337
