HA-hDAGLb-V5
(Plasmid #87675)

• Purpose: Expresses human DAGLb protein with a HA tag inserted in the first extracellular loop, and intracellular V5 tag located on the C-terminal
• Depositing Lab: Pat Doherty
• Publication: Unpublished Doherty Lab Plasmids (unpublished)

Backbone

• Vector backbone: pcDNA6.2-DEST/V6
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 7341
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Human Diacylglycerol Lipase Beta
• Alt name: hDAGLb
• Species: H. sapiens (human)
• Insert Size (bp): 2070
• Mutation: Hemagglutinin (HA) peptide sequence (YPYDVPDYA) flanked by a glycine-serine-glycine-serine linker on either side was inserted into the first extracellular loop of Human DAGLb sequence
• Entrez Gene: DAGLB (a.k.a. DAGLBETA, KCCR13L)
• Promoter: T7
• Tags / Fusion Proteins:
  • V5 tag (C terminal on backbone)
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: V5 Rev Priming Site

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Derived from hDAGLb-V5 construct.

This construct carries a Q664R mutation compared to the NCBI reference sequence NM_139179. This mutation is a natural variant and should not affect plasmid function.