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Addgene

RV-Syn-Dio-GFP
(Plasmid #87673)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87673 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    RV-Syn
  • Vector type
    Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eGFP
  • Insert Size (bp)
    733
  • Promoter Synapsin

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer WPRE-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

subclone from RV-cag-dio-GFP, removal of CAG promoter and replace with Synapsin

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RV-Syn-Dio-GFP was a gift from Oscar Marin (Addgene plasmid # 87673 ; http://n2t.net/addgene:87673 ; RRID:Addgene_87673)

  • For your References section:

    Optimization of interneuron function by direct coupling of cell migration and axonal targeting. Lim L, Pakan JMP, Selten MM, Marques-Smith A, Llorca A, Bae SE, Rochefort NL, Marin O. Nat Neurosci. 2018 Jun 18. pii: 10.1038/s41593-018-0162-9. doi: 10.1038/s41593-018-0162-9. 10.1038/s41593-018-0162-9 PubMed 29915195
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