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Addgene

T7T3-PacI-Neto1
(Plasmid #87672)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87672 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pT7T3D-PacI
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Neto1
  • Alt name
    NM_144946.4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1034
  • Mutation
    partial sequence, (1244 to 2278bp of NM_144946.4)
  • Entrez Gene
    Neto1 (a.k.a. Btcl1, AI851453, C130005O10Rik)
  • Promoter T7/T3

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7p
  • 3′ sequencing primer T3p
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    T7T3-PacI-Neto1 was a gift from Oscar Marin (Addgene plasmid # 87672 ; http://n2t.net/addgene:87672 ; RRID:Addgene_87672)

  • For your References section:

    Optimization of interneuron function by direct coupling of cell migration and axonal targeting. Lim L, Pakan JMP, Selten MM, Marques-Smith A, Llorca A, Bae SE, Rochefort NL, Marin O. Nat Neurosci. 2018 Jun 18. pii: 10.1038/s41593-018-0162-9. doi: 10.1038/s41593-018-0162-9. 10.1038/s41593-018-0162-9 PubMed 29915195
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