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T7T3-PacI-elfn1_459to1199
(Plasmid #87671)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87671 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pT7T3D-PacI
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Elfn1
  • Alt name
    NM_175522.3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    641
  • Mutation
    partial sequence, (459 to 1199bp off NM_175522.3)
  • Entrez Gene
    Elfn1 (a.k.a. AI197272, A930017N06Rik)
  • Promoter T7/T3

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7p
  • 3′ sequencing primer T3p
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    T7T3-PacI-elfn1_459to1199 was a gift from Oscar Marin (Addgene plasmid # 87671 ; http://n2t.net/addgene:87671 ; RRID:Addgene_87671)

  • For your References section:

    Optimization of interneuron function by direct coupling of cell migration and axonal targeting. Lim L, Pakan JMP, Selten MM, Marques-Smith A, Llorca A, Bae SE, Rochefort NL, Marin O. Nat Neurosci. 2018 Jun 18. pii: 10.1038/s41593-018-0162-9. doi: 10.1038/s41593-018-0162-9. 10.1038/s41593-018-0162-9 PubMed 29915195
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