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Addgene

pBluescript-KS-Nrg3-ISH
(Plasmid #87669)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87669 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Pbluescript-KS
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nrg3
  • Alt name
    AF010130.1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    500
  • Entrez Gene
    Nrg3 (a.k.a. ska)
  • Promoter T7/M13

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BstB1 (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBluescript-KS-Nrg3-ISH was a gift from Oscar Marin (Addgene plasmid # 87669 ; http://n2t.net/addgene:87669 ; RRID:Addgene_87669)

  • For your References section:

    Neuregulin 3 Mediates Cortical Plate Invasion and Laminar Allocation of GABAergic Interneurons. Bartolini G, Sanchez-Alcaniz JA, Osorio C, Valiente M, Garcia-Frigola C, Marin O. Cell Rep. 2017 Jan 31;18(5):1157-1170. doi: 10.1016/j.celrep.2016.12.089. 10.1016/j.celrep.2016.12.089 PubMed 28147272
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