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Addgene

RV-cag-Dio-Kif5c-2A-tdt
(Plasmid #87668)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87668 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    RV-Syn
  • Vector type
    Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kif5C-P2A-mTdt
  • Alt name
    NM_001107730.1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    4002
  • Mutation
    motor domain aa1-560 present, V40A, E125V (see depositor comment below)
  • Entrez Gene
    Kif5c
  • Promoter CAG
  • Tag / Fusion Protein
    • palmitylation (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer pRRL-Fwd
  • 3′ sequencing primer WPRE-R
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that several discrepancies (including mutation N185S in EYFP, and mutations V40A, E125V in Kif5C) were found between Addgene's quality control and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

Kif5Cdelta560 subclone from addgene plasmid 45059, pBa.Kif5c 1-560.GFP

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RV-cag-Dio-Kif5c-2A-tdt was a gift from Oscar Marin (Addgene plasmid # 87668 ; http://n2t.net/addgene:87668 ; RRID:Addgene_87668)

  • For your References section:

    Optimization of interneuron function by direct coupling of cell migration and axonal targeting. Lim L, Pakan JMP, Selten MM, Marques-Smith A, Llorca A, Bae SE, Rochefort NL, Marin O. Nat Neurosci. 2018 Jun 18. pii: 10.1038/s41593-018-0162-9. doi: 10.1038/s41593-018-0162-9. 10.1038/s41593-018-0162-9 PubMed 29915195
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