RV-Cag-Dio-mTdt
(Plasmid
#87665)
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Purposeretroviral vector encoding cre dependent expression of membrane Tdtomato
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 87665 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneRV-CAG
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Vector typeRetroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMem-Tdtomato
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Insert Size (bp)1499
- Promoter CAG
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PacI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer pRRL-Fwd
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
subclone from plasmid RV-cag-dio-GFP
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RV-Cag-Dio-mTdt was a gift from Oscar Marin (Addgene plasmid # 87665 ; http://n2t.net/addgene:87665 ; RRID:Addgene_87665) -
For your References section:
Optimization of interneuron function by direct coupling of cell migration and axonal targeting. Lim L, Pakan JMP, Selten MM, Marques-Smith A, Llorca A, Bae SE, Rochefort NL, Marin O. Nat Neurosci. 2018 Jun 18. pii: 10.1038/s41593-018-0162-9. doi: 10.1038/s41593-018-0162-9. 10.1038/s41593-018-0162-9 PubMed 29915195