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Addgene

3279 pSFFV-neo Bid human
(Plasmid #8766)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8766 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSFFV-neo
  • Backbone size w/o insert (bp) 7200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bid ORF cDNA FL
  • Alt name
    Bid
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1200
  • Entrez Gene
    BID (a.k.a. FP497)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SFFV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3279 pSFFV-neo Bid human was a gift from Stanley Korsmeyer (Addgene plasmid # 8766 ; http://n2t.net/addgene:8766 ; RRID:Addgene_8766)

  • For your References section:

    BID: a novel BH3 domain-only death agonist. Wang K, Yin XM, Chao DT, Milliman CL, Korsmeyer SJ. Genes Dev 1996 Nov 15;10(22):2859-69. 10.1101/gad.10.22.2859 PubMed 8918887
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