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Addgene

pNCMV HPV8 E6 K64A
(Plasmid #87656)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87656 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CMV Bam neo
  • Backbone size w/o insert (bp) 6550
  • Total vector size (bp) 7100
  • Modifications to backbone
    Flag/HA containing added at BamHI site
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HPV 8E6
  • Species
    HPV
  • Insert Size (bp)
    542
  • Mutation
    K64A
  • GenBank ID
    M12737.1
  • Promoter CMV
  • Tags / Fusion Proteins
    • Flag (N terminal on backbone)
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer Bglobin intron F
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The 8E6 template was given to us by Harold Pfister who originally cloned the HPV8 genome. (J. Virology 1986, Pfister H. et al.)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNCMV HPV8 E6 K64A was a gift from Karl Munger (Addgene plasmid # 87656 ; http://n2t.net/addgene:87656 ; RRID:Addgene_87656)

  • For your References section:

    Cutaneous HPV8 and MmuPV1 E6 Proteins Target the NOTCH and TGF-beta Tumor Suppressors to Inhibit Differentiation and Sustain Keratinocyte Proliferation. Meyers JM, Uberoi A, Grace M, Lambert PF, Munger K. PLoS Pathog. 2017 Jan 20;13(1):e1006171. doi: 10.1371/journal.ppat.1006171. PPATHOGENS-D-16-02140 [pii] PubMed 28107544
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