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Addgene

UAS-Syt::Halo7
(Plasmid #87648)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87648 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJFRC81
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Syt::Halo7
  • Species
    Synthetic
  • Mutation
    S265G in Syt (please see depositor's comment below)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GenBank accession number: KY511558

Please note that mutation S265G in Syt was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UAS-Syt::Halo7 was a gift from Gregory Jefferis (Addgene plasmid # 87648 ; http://n2t.net/addgene:87648 ; RRID:Addgene_87648)

  • For your References section:

    Second-Generation Drosophila Chemical Tags: Sensitivity, Versatility, and Speed. Sutcliffe B, Ng J, Auer TO, Pasche M, Benton R, Jefferis GS, Cachero S. Genetics. 2017 Apr;205(4):1399-1408. doi: 10.1534/genetics.116.199281. Epub 2017 Feb 16. 10.1534/genetics.116.199281 PubMed 28209589
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