3269 pBS Bcl-2
(Plasmid #8764)

• Depositing Lab: Stanley Korsmeyer
• Publication: Silverman et al Proc Natl Acad Sci U S A 1990 Dec;87(24):9913-7.

Backbone

• Vector backbone: pBluescript KS
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Bcl-2
• Species: M. musculus (mouse)
• Insert Size (bp): 600
• Entrez Gene: Bcl2 (a.k.a. Bcl-2, C430015F12Rik, D630044D05Rik, D830018M01Rik)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Main use: in situ probe. Cut with HindIII and use T7 polymerase for antisense probe. Cut with XbaI and use T3 for sense probe.