Tau (P301L)-VC
(Plasmid #87633)

• Purpose: expresses hTau fused to the C-terminal part of Venus
• Depositing Lab: Tiago Outeiro
• Publication: Unpublished plasmid (unpublished)

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5691
• Total vector size (bp): 7013
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MAPT
• Alt name: Tau
• Species: H. sapiens (human)
• Insert Size (bp): 1322
• Mutation: Mutation P301L
• GenBank ID: NM_005910.5
• Entrez Gene: MAPT (a.k.a. DDPAC, FTD1, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40)
• Promoter: CMV
• Tag / Fusion Protein:
  • N-terminal part of Venus (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AflII (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: bGH

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Original clone (alpha-Synuclein-VC) comes from Pamela J.McLean.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Original clone (alpha Synuclein-VC) includes already the C-terminal part of venus, and was a gift from Pamela J.McLean.