pRVV647
(Plasmid
#87630)
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PurposeDonor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked alleles.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 87630 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescript SK+
- Total vector size (bp) 11192
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBxb1 attP
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SpeciesMycobacterium phage Bxb1
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRVV647 was a gift from Richard Mann (Addgene plasmid # 87630 ; http://n2t.net/addgene:87630 ; RRID:Addgene_87630) -
For your References section:
Bxb1 phage recombinase assists genome engineering in Drosophila melanogaster. Voutev R, Mann RS. Biotechniques. 2017 Jan 1;62(1):37-38. doi: 10.2144/000114494. 10.2144/000114494 PubMed 28118814
Map uploaded by the depositor.
