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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8763 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescript KS
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
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Vector typeCloning vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMcl-1
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SpeciesM. musculus (mouse)
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Insert Size (bp)1700
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GenBank IDNM_008562
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Entrez GeneMcl1 (a.k.a. Gm52627, Mcl-1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
EcoRI is 5' end of cDNA. SacII, NotI, XbaI, EcoRI, EcoRV, ClaI, SalI, Asp718/KpnI do not cut in cDNA.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
3265 pBS Mcl-1 was a gift from Stanley Korsmeyer (Addgene plasmid # 8763 ; http://n2t.net/addgene:8763 ; RRID:Addgene_8763) -
For your References section:
Mcl-1 deficiency results in peri-implantation embryonic lethality. Rinkenberger JL, Horning S, Klocke B, Roth K, Korsmeyer SJ. Genes Dev 2000 Jan 1;14(1):23-7. PubMed 10640272
Map uploaded by the depositor.
